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Copyright © 2006, CMBS Smith College |
Getting started in the CMB- HHMI scheduler
Sanger Sequencing
How to set up a sequencing reaction and product recommendations
Things you can do with your own DNA
DNA Isolation from Cheek Cells
DNA isolation from animal saliva using the DNeasy Blood and Tissue Kit by Qiagen
Creating a Chromosome Spread From Blood
Creating a Karyotype from your Chromosome Spread with Photoshop
Transilluminators
User guide to transilluminators
Microarrays
cDNA: Synthesis using Genisphere, labelling, and hybridization to a microarray - CMB
cDNA: Synthesis using Genisphere, labelling, and hybridization to a microarray - CWZ lab
How-to-Use Guide for GenePix 4100 Scanner and 4.1 Software (powerpoint)
Microarray analysis using MeV Tutorial
Quantitative Real Time PCR
Notes for the StepOne Plus system and designing your QRT PCR Experiment
Spectrophotometry
How-to-use guide for the M5 plate reader and SpectraMax Software in the CMB
Quick Guide to the plate reader
How to export results files from the plate reader into Excel
How-to-use guide for the Nanodrop Spectrophotometer
Guidelines to using the Qubit Flourimeter
Protocol for Qubit DNA BR Assay
Protocol for Qubit DNA HS Assay
Protocol for Qubit Protein Assay
Protocol for Qubit ssDNA Assay
If you have a protocol you would like to share with others, or if there is a protocol you would like to see on this page, please contact the CMB's ITI, Louie Bierwert, at lbierwer@smith.edu