Consultation

• Experimental design and planning
• Proper sample preparation for subsequent MS-based analysis
• Assistance with data interpretation

Assistance with sample preparation

(please note that the CFP does not provide reagents, kits or other materials)         

• Sample clean-up and processing (extraction, desalting, precipitation, depletion, fractionation, proteolytic digest, etc.)
• Standard biochemical techniques (SDS-PAGE gel electrophoresis and staining, Western blotting, etc.)
• Affinity-based enrichment (antibody, immobilized metal affinity, etc.)

General Mass Spectrometry

Small Molecules (see Protocols for appropriate sample preparation):

• Electrospray ionization of acidic, basic, charged and polar molecules (direct infusion)
• Reverse phase liquid chromatography hyphenation (other stationary phases possible, must be supplied by researcher)
• MS and MS/MS
• Positive and negative ion detection
• Mass determination and quantitation (when standards are available)

Metabolomics:

• Untargeted and Targeted workflows
• Metabolite identification and quantitation
• Data visualization and analysis (molecular networks, heat maps, volcano plots, etc)

Proteomics:

• Protein and peptide identification in purified samples or complex mixtures
• Protein Quantitation: Isobaric tags
• PTM identification
• Intact protein analysis

Data Analysis

• See currently available software packages

Custom Data Analysis Scripts

• Development of custom data processing and/or visualization scripts in Python, either as standalone projects or as feature requests for our in-house software MPACT.