Mass Spectrometry and Chromatography at Smith College
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Consultation
Experimental design and planning
Proper sample preparation for subsequent MS-based analysis
Assistance with data interpretation
Assistance with sample preparation
(please note that the CFP does not provide reagents, kits or other materials)
Sample clean-up and processing (extraction, desalting, precipitation, depletion, fractionation, proteolytic digest, etc.)
Standard biochemical techniques (SDS-PAGE gel electrophoresis and staining, Western blotting, etc.)
Affinity-based enrichment (antibody, immobilized metal affinity, etc.)
General Mass Spectrometry
Small Molecules (see Protocols for appropriate sample preparation):
Electrospray ionization of acidic, basic, charged and polar molecules (direct infusion)
Reverse phase liquid chromatography hyphenation (other stationary phases possible, must be supplied by researcher)
MS and MS/MS
Positive and negative ion detection
Mass determination and quantitation (when standards are available)
Metabolomics:
Untargeted and Targeted workflows
Metabolite identification and quantitation
Data visualization and analysis (molecular networks, heat maps, volcano plots, etc)
Proteomics :
Protein and peptide identification in purified samples or complex mixtures
Protein Quantitation: Isobaric tags
PTM identification
Intact protein analysis
Data Analysis
Custom Data Analysis Scripts
Development of custom data processing and/or visualization scripts in Python, either as standalone projects or as feature requests for our in-house software MPACT.