Biological Sciences 300, Smith College | NeurophysiologySchedule, Spring 2020http://www.science.smith.edu/departments/NeuroSci/courses/bio330/syllabus.html [also: tinyurl.com/bio300] UPDATED: February 20, 2020 |
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Bio 300 Home | Schedule | Videos | Administrative Information |
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The 2017 syllabus remains available online. It contains links to laboratory exercises, |
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2020 DATES |
TOPICS AND ASSIGNMENTS COMPRESS SCHEDULE |
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Jan 28-30 |
ELECTRICAL SIGNALS IN NEURONS Neurons convey information: sensory receptors for touch. Rapidly-adapting (phasic) vs. slowly-adapting (tonic)
responses. Case discussion: How accurate is sensory reality? Example: crustacean muscle receptor organ (MRO or
"stretch receptor").
Visualizing brains and neurons. The unfixed brain (6:19)Visualizing individual neurons: general stains, injecting fluorescent dye or dense markers, HRP backfill, lipid-soluble dyes, immunocytochemistry, genetically expressed fluorescent proteins, "brainbow," CLARITY technique, connectomics.
Collaborative Writing Project
Chapter 1 ends here. DUE: Feb 6. |
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Feb 4-6 |
MEMBRANE POTENTIALS Ions, pumps, and membrane potentials. Squid giant axon. Distribution of ions in axon and blood. Na/K pump: active transport of ions. Case discussion: Why do red blood cells need pumps? Forces acting on ions. Equilibrium between diffusion and electrical attraction. Equilibrium potential. Nernst equation. Ions that can cross membrane carry charge until cell's potential matches the ion's equilibrium potential. Concentrations can be regarded as constant. Preview of the action potential.
Membrane channels for Na+ and K+ ions.Voltage clamping: command and measured potentials;
inject current as needed to maintain "clamped" potential. Separating currents due to Na ions and K ions (low-Na, TTX, TEA). Na-inactivation. Case discussion: A lethal shipboard snack. Calculating conductance for each ion. Peak conductance vs. potential. Peak current vs. potential.
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Feb 11-13 |
Voltage clamping (continued) Reconstructing the action potential from voltage-clamp
data. Threshold and refractory period. Patch clamping to look at individual channels.
Collaborative Writing Project Chapter 2 ends here. DUE: Feb 18.
Propagation of the action potential. Local circuit currents. Length constant. Conduction velocity. Strategies for faster conduction: giant fibers and myelination. Demyelinating diseases. Case discussion: The case of the missing channels.
Collaborative Writing Project Chapter 3 (covering only one class) ends here. DUE: Feb 20. |
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Feb 18-20 |
Generator channels. Other voltage-dependent channels. "Generator-type" channels: not electrically excitable. Examples: stretch-activated channels. Reversal potential suggests small positive ions go through the channels. Initiation of action potentials at nearest low-threshold site. Calcium and potassium channels (IA, IC) that modulate firing rate of neurons. Optogenetic channels. Optical recording methods.
Molecular structure of voltage-dependent channels. Physiological and genetic insights to structure of membrane channels. TTX binding at selectivity filter, pronase attack on inactivation gate. Purification of Na channel protein, sequencing of gene. Deductions about structure and function, S4 helix as probable voltage sensor. Solving the molecular structure of the bacterial KcsA channel. Location of selectivity filter. Structure of the voltage-gated Shaker channel. Evolution of Ca and Na channels: four domains resembling Shaker channel.
Collaborative Writing Project Chapter 4 ends here. DUE: Feb 27
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Feb 25-27 |
Feb 25: MetaNeuron simulations.
Class meets in 408 Sabin-Reed. Feb 27: Review Q&A. |
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Mar 3 |
EXAM on membrane potentials, in class (topics through Feb 27
classes and readings). |
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Mar 5 |
SYNAPSES Electrical synapses:
structure of gap junctions, examples of electrical
conduction.
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Mar 10-12 |
Presynaptic release
of vesicles. Endplate potential. "Minepps." Quantal
release.. Postsynaptic receptors for
acetylcholine. ACh degradation: acetylcholine esterase. Synthesis: choline acetyl transferase. Reuptake and repackaging in vesicles via transporters. Pharmacology of the neuromuscular junction.
Collaborative Writing Project Chapter 5 ends here. DUE: Mar 26. |
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Mar 14-22 |
Spring break |
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Mar 24-26 |
Neuron-to-neuron synapses:
spinal motoneurons. Transmitters activating second messengers (metabotropic receptors). Classical ionotropic (fast) vs. metabotropic receptors
(slow). Example: autonomic nervous system. Mechanisms of action: collision-coupling to channels, second messengers causing phosphorylation of channels, opening or closing channels. Modulation at synapses: Multiple second-messenger systems, overlapping pathways, pre- and post-synaptic modulation.
Collaborative Writing Project Chapter 6 ends here. DUE: Apr 2. |
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Mar 31 |
GENERATING MOVEMENT. Levels of control: within muscle cells (graded depolarization and calcium levels). Control at the motor unit (firing frequency and recruitment). Feedback from spindles and Golgi tendon organs.
Central control of posture and locomotion: command interneurons in crayfish, central pattern generators for locomotion in Tritonia, crayfish, roaches and cats. Role of sensory feedback in CPGs. Case discussion: Swimming blindly (based on your response papers -- see the Special Assignment below).
Collaborative Writing Project Chapter 7 ends here. DUE: Apr 9.
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Apr 7-9 |
VISION. Structure of eyes and retinas. This introductory topic will be covered by two online lessons hosted on our Moodle site. Please view these lessons before Tuesday's class. The remaining topics will be covered in class: The retina. Visual pigments, responses of photoreceptors to light. Synaptic network. Horizontal cells. Center-surround receptive fields of bipolar cells. Retinal ganglion cells. Transient (Y)
and sustained (X) ganglion cells. Spatial distribution of retinal ganglion cells.
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Apr 14-16 |
Visual pathway: lateral geniculate nucleus and visual cortex. Simple, complex and "hypercomplex" cells in striate cortex.
Primary visual cortex. Binocular (stereo) vision. Computer-based receptive field mapping. Spatial frequency selectivity.
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Apr 21-23 |
Cortical anatomy:
Retinotopic map, ocular dominance columns, orientation pinwheels, spatial
frequency patches, blobs, cortical layers.
Extrastriate cortex: Pathways for motion and form. Dorsal pathway, area MT, direction and disparity selectivity.
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Apr 28-30 |
Inferotemporal cortex: Ventral pathway to inferotemporal lobe. Objects and faces. Visual perception. Top-down and bottom-up components of visual perception. Case discussion: Signaling by a face-selective neuron.
Color vision: retina, LGN, V1. Color patches and blobs. V4: color constancy.
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TBA |
Cumulative review session (optional). Bring questions! |
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May 5-8 |
Final exam, self-scheduled. |
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