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Optimized strategy for real-time qPCR detection of Onchocerca volvulus DNA in pooled Simulium sp . blackfly vectors. Draft Genome of the Polynesian Tiger Mosquito, Aedes polynesiensis. Grant, J.R., Bushko, R., Chertock, S., Gonzalez, A., Halili, S., Hwang, S., Mathieu-Daudé, F., Morgan, R., Newhall, R., Oprea, E., Pietrow, R., Stauduhar, J., Sun, L., Wang, H., Bossin, H., Slatko, B., Williams, S.A. (in review, 2021). Diagnostics to support elimination of lymphatic filariasis-Development of two target product profiles. Development of a novel real-time polymerase chain reaction assay for the sensitive detection of Schistosoma japonicum in human stool. Parasitic Disease Surveillance, Mississippi, USA. CRISPR/Cas9-based genome editing of the Saccharomyces cerevisiae ADE2 gene with restriction-free cloning and a rapid BamHI digest readout. Molecular detection of intestinal helminths and protozoa among young children in Dosso Region, Niger. Laboratory evaluation of molecular xenomonitoring using mosquito and tsetse fly excreta/feces to amplify Plasmodium, Brugia, and Trypanosoma DNA Parasitic Infection Surveillance in Mississippi Delta Children. First international external quality assessment scheme of nucleic acid amplification tests for the detection of Schistosoma and soil-transmitted helminths, including Strongyloides: A pilot study. Rectal Swabs as an Alternative Sample Collection Method to Bulk Stool for the Real-Time PCR Detection of Giardia duodenalis. A Novel Quantitative Real-Time PCR Diagnostic Assay for Fecal and Nasal Swab Detection of an Otariid Lungworm, Parafilaroides decorus. Williams KM, Fessler MK, Bloomfield RA, Sandke WD, Malekshahi CR, Keroack CD, Duignan PJ, Torquato SD, Williams SA. Int J Parasitol Parasites Wildl. 2020;12:85‐92. Published 2020 May 18. doi:10.1016/j.ijppaw.2020.04.012Selection and exploitation of prevalent, tandemly repeated genomic targets for improved real-time PCR-based detection of Wuchereria bancrofti and Plasmodium falciparum in mosquitoes. Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. Field evaluation of DNA detection of human filarial and malaria parasites using mosquito excreta/feces Semester-Long Course-Based Research Project in Second-Semester Organic Chemistry: Synthesizing Potential Lead Compounds for the Treatment of a Neglected Tropical Disease. What does soil-transmitted helminth elimination look like? Results from a targeted molecular detection survey in Japan . A case for using genomics and a bioinformatics pipeline to develop sensitive and species-specific PCR-based diagnostics for soil-transmitted helminths. Pooling as a strategy for the timely diagnosis of soil-transmitted helminths in stool: value and reproducibility. Targeting a highly repeated germline DNA sequence for improved real-time PCR-based detection of Ascaris infection in human stool. Effects of single and integrated water, sanitation, handwashing, and nutrition interventions on child soil-transmitted helminth and Giardia infections: A cluster-randomized controlled trial in rural Kenya. Causative agent of canine heartworm (Dirofilaria immitis) detected in wild lemurs. Backpack PCR: A point-of-collection diagnostic platform for the rapid detection of Brugia parasites in mosquitoes. Ancylostoma ceylanicum Hookworm in Myanmar Refugees, Thailand, 2012-2015. A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus). A Comparative Analysis of Preservation Techniques for the Optimal Molecular Detection of Hookworm DNA in a Human Fecal Specimen. A Superhydrophobic Cone to Facilitate the Xenomonitoring of Filarial Parasites, Malaria, and Trypanosomes Using Mosquito Excreta/Feces. The Current Status of Molecular Xenomonitoring for Lymphatic Filariasis and Onchocerciasis. Pilotte N, Unnasch TR, Williams SA. A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite Ancylostoma ceylanicum in human stool. Application of a household-based molecular xenomonitoring strategy to evaluate the lymphatic filariasis elimination program in Tamil Nadu, India. Profiling the Macrofilaricidal Effects of Flubendazole on Adult Female Brugia malayi using RNAseq. The Effects of Ivermectin on Brugia malayi Females In Vitro: A Transcriptomic Approach. Improved PCR-Based Detection of Soil Transmitted Helminth Infections Using a Next-Generation Sequencing Approach to Assay Design .A Novel Xenomonitoring Technique Using Mosquito Excreta/Feces for the Detection of Filarial Parasites and Malaria .Absence of the Filarial Endosymbiont Wolbachia in Seal Heartworm (Acanthocheilonema spirocauda) but Evidence of Ancient Lateral Gene Transfer. Phenotypic and Molecular Analysis of the Effect of 20-hydroxyecdysone on the Human Filarial Parasite Brugia malayi The Effect of In Vitro Cultivation on the Transcriptome of Adult Brugia malayi Time for T? Immunoinformatics addresses vaccine design for neglected tropical and emerging infectious diseases Molecular xenomonitoring using mosquitoes to map lymphatic filariasis after mass drug administration in American Samoa A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi A multicenter evaluation of diagnostic tools to define endpoints for programs to eliminate bancroftian filariasis The NIH-NIAID Filariasis Research Reagent Resource Center .A Community-Based Study of Factors Associated with Continuing Transmission of Lymphatic Filariasis in Leogane, Haiti Detection of Wuchereria bancrofti L3 Larvae in Mosquitoes: A Reverse Transcriptase PCR Assay Evaluating Infection and Infectivity Immunisation with a Multivalent, Subunit Vaccine Reduces Patent Infection in a Natural Bovine Model of Onchocerciasis during Intense Field Exposure Heritable factors play a major role in determining host responses to Wuchereria bancrofti infection in an isolated South Pacific island population The Impact of Repeated Rounds of Mass Drug Administration with Diethylcarbamazine Plus Albendazole on Bancroftian Filariasis in Papua New Guinea A Reverse Transcriptase-PCR Assay for Detecting Filarial Infective Larvae in Mosquitoes Determinants of Success in National Programs to Eliminate Lymphatic Filariasis: a Perspective Identifying Essential Elements and Research Needs Human Cardiac-Specific cDNA Array for Idiopathic Dilated Cardiomyopathy: Sex-Related Differences An Unidentified Filarial Species and its Impact on Fitness in Wild Populations of the Black-Footed Ferret (Mustela nigripes) Draft Genome of the Filarial Nematode Parasite Brugia malayi Seroprevalence of Dengue in Trinidad Using Rapid Test Kits: A Cord Blood Survey A Real-Time PCR-based Assay for Detection of Wuchereria bancrofti DNA in Blood and Mosquitoes Characterization of a Novel Filarial Serine Protease Inhibitor, Ov-SPI-1, from Onchocerca volvulus, with Potential Multifunctional Roles During Development of the Parasite .Profiling of Gender-Regulated Gene Transcripts in the Filarial Nematode Brugia malayi by cDNA Oligonucleotide Array Analysis .Filarial Genomics . S.A. Williams. Am. J. Trop. Med. Hyg., S71: 37-40, 2004.Towards a strategic plan for research to support the global program to eliminate lymphatic filariasis. Summary of Immediate needs and opportunities for research on lymphatic filariasis Development and Standardization of a Rapid, PCR-based Method for the Detection of Wuchereria bancrofti in Mosquitoes, for Xenomonitoring the Human Prevalence of bancroftian filariasis .The River Blindness Genome Project Filarial Genomics: Gene Discovery and Gene Expression Xenomonitoring of Culex quinquefasciatus Mosquitoes as a Guide for Detecting the Presence or Absence of Lymphatic Filariasis: a Preliminary Protocol for Mosquito Sampling .Quantification of PCR Amplification Products of Brugia HhaI Repeat DNA Using a Semiautomated Q-PCR System Evaluation of SspI Polymerase Chain Reaction Assay in the Detection of Wuchereria bancrofti Infection in Field-Collected Culex quinquefasciatus and its Application in the Transmission Studies of Lymphatic Filariasis . S.L. Hoti, K.P. Patra, V. Vasuki, M.W. Lizotte, V.R. Hariths, N. Sushma, K. Gunasekaran, K.D. Ramaiah, P. Vanamail, T. Mariappan, and S.A. Williams. Journal of Applied Entomology-Zeitschrift fur Ange Wandte Entomologie. 126: 417-421, 2002.Rapid PCR-based Detection of Brugia malayi DNA from Blood Spots by DNA Detection Test Strips .Detection of Brugia malayi in Laboratory and Wild-caught Mansonioides Mosquitoes (Diptera: Culicidae) Using HhaI PCR Assay. Application of a Polymerase Chain Reaction-ELISA to Detect Wuchereria bancrofti in Pools of Wild-Caught Anopheles punctulatus in a Filariasis Control Area in Papua New Guinea. Identification of Potential Vaccine and Drug Target Candidates by Expressed Sequence Tag Analysis and Immunoscreening of Onchocerca volvulus Larval cDNA Libraries. The Filarial Genome Project: Analysis of the Nuclear, Mitochondrial and Endosymbiont Genomes of Brugia malayi The Genomes of Onchocerca volvulus Detection of DNA of Nocturnally Periodic Brugia malayi in Night and Day Blood Samples by a Polymerase Chain Reaction-ELISA-Based Method Using an Internal Control DNA Helminth Genome Analysis: the Current Status of the Filarial and Schistosome Genome Projects . S. A. Williams and D.A. Johnston. Parasitology, 118: S19-S38, 1999.Patterns of Onchocerca volvulus Recombinant Antigen Recognition in a Bovine Model of Onchocerciasis Diagnosis of Wuchereria bancrofti Infection by the Polymerase Chain Reaction Employing Patient’s Sputum Chemiluminescent Detection of Sequential DNA Hybridizations to High Density, Filter Arrayed cDNA Libraries: a Subtraction Method for Novel Gene Discovery Deep Within the Filarial Genome: an Update on the Progress of the Filarial Genome Project Development of a Quantitative, Competitive Polymerase Chain Reaction-Enzyme-Linked Immunosorbent Assay for the Detection of Wuchereria bancrofti DNA PCR-ELISA for the Detection of Brugia malayi Infection Using Finger-Prick Blood Detection of the Filarial Parasite Mansonella streptocerca in Skin Biopsies by a Nested Polymerase Chain Reaction-Based Assay Thioredoxin Peroxidase (TPx) from Onchocerca volvulus: a Major Hydrogen Peroxide Detoxifying Enzyme in Filarial Parasites The immunodominant Brugia malayi paramyosin as a marker of current infection with Wuchereria bancrofti adult worms A Unified Nomenclature for Filarial Genes The Filarial Genome Project . Blaxter, M.L., J. Daub, M. Waterfall, D. Guiliano, S.A. Williams, K. Jayaraman, R. Ramzy, B. Slatko and A. Scott. COST 819, The Commission of the European Community, Brussels, 1997.A Polymerase Chain Reaction-Based Assay for Detection of Wuchereria bancrofti in Human Blood and Culex pipiens Detection of Wuchereria bancrofti DNA in Patients’ Sputum by the Polymerase Chain Reaction Evaluation of a Polymerase Chain Reaction-Based Assay for Diagnosis of Wuchereria bancrofti Infection A Polymerase Chain Reaction Assay for the Detection of Wuchereria bancrofti in Blood Samples from French Polynesia Parasite Genome Projects . S.A. Williams and D.J. Kemp. In: The Encyclopedia of Molecular Biology and Molecular Medicine, VCH Publishers Inc., New York, NY. p. 306-312, 1996.Identification of Novel Sequences and Codon Usage in Strongyloides stercoralis A Cyclosporin A-sensitive Small Molecular Weight Cyclophilin of Filarial Parasites Genes Expressed in Brugia malayi Infective Third Stage Larvae A PCR Assay for the Detection of Wuchereria bancrofti in Blood Chitinase Genes Expressed by Infective Larvae of the Filarial Nematodes, Acanthocheilonema vitae and Onchocerca volvulus .Detection of Wuchereria bancrofti Larvae in Pools of Mosquitoes by the Polymerase Chain Reaction Construction of cDNA Libraries from Limiting Amounts of Material A Polymerase Chain Reaction Assay for the Detection of Brugia malayi in blood Molecular Phylogenetic Studies on Filarial Parasites Based on 5S Ribosomal Spacer Sequences Molecular Phylogenetic Studies of the Genus Brugia. H. Xie, O. Bain, and S.A. Williams. Parasite, 1: 255-260, 1994.
Brugia malayi and Brugia pahangi: Synthetic Biotin Labeling of Oligonucleotide Probes for Use in Species-Specific Detection Assays Recent Advances in the Application of Molecular Biology in Filariasis A Proline-Rich Structural Protein of the Surface Sheath of Larval Brugia Filarial Nematode Parasites Diagnosis of Filariasis with DNA Probes Filarial DNA Probes in Jakarta . L.A. McReynolds, C.B. Poole, and S.A. Williams. Parasitology Today, 7: 65-67, 1991.A Rapid DNA Assay for the Species-Specific Detection and Quantification of Brugia in Blood Samples A Field Study Using the Polymerase Chain Reaction (PCR) to Screen for Brugia Microfilariae in Human and Animal Blood Detection of Brugia malayi and Brugia pahangi Parasites by Biotinylated DNA Probes Species-Specific Oligonucleotide Probes for the Identification of Human Filarial Parasites Development of DNA Probes to Identify and Speciate Filarial Parasites Highly Repeated DNA Sequences as Species-Specific Probes for Brugia The Use of DNA Sequencing Reactions as Probes for Southern Blots, Dot Blots and Colony Hybridizations . C.O. Card, S.A. Williams, L.S. Moran, C.B. Poole, and B.E. Slatko. Biotechniques, 5: 302-307, 1987.The Genomic Complexity and Tubulin Gene of Dirofilaria immitis . C.V. Maina, A.G. Grandea, L.T.K. Tuyen, N. Asikin, S.A. Williams, and L.A. McReynolds. In “Molecular Paradigms for Eradicating Helminthic Parasites” (A. MacInnis, Ed.). Alan R. Liss, Inc., New York, NY, 1987.Sequencing in the Fast Lane: a Rapid Protocol for [35S]-dATP Dideoxy DNA Sequencing . S.A. Williams, B.E. Slatko, L.S. Moran, and S.M. DeSimone. Biotechniques, 4: 138-147, 1986.Cloning and Comparison of Repeated DNA Sequences from the Human Filarial Parasite Brugia malayi and the Animal Parasite Brugia pahangi Cloning the Gene for the Malolactic Fermentation of Wine from Lactobacillus delbrueckii in Escherichia coli and Yeasts Qualitative and Quantitative Differences in Learning Associated with Multiple-Choice Testing
