TITLE:  The Collection of Brugia sp. from Jirds, and the Surgical Transplantation of Adult

              Brugia sp. into Jirds

SOP NUMBER:  8.94

REVISION NUMBER:  0

1.0       PURPOSE

The purpose of this document is to describe the procedure used when necropsying jirds for the collection of Brugia sp. and to describe the procedure used when transplanting adult Brugia sp. intraperitoneally into recipient jirds.

2.0       PROCEDURE

2.1       Collection of Brugia sp.

2.1.1     Prior to necropsy, euthanize the jird(s) by exposure to CO_2.

2.1.2     Shave the abdominal area of the jird(s).

2.1.3     Examine the peritoneal cavity and remove the Brugia sp. worms.

2.1.4     Dissect the heart, lungs, and testes and remove the worms.

2.1.5    Place all of the worms from the jird(s) in TC Hank=s balanced salt solution containing an appropriate antibiotic such as penicillin/streptomycin.

2.2       Surgical Transplantation of Brugia sp.Into the Peritoneal Cavity

2.2.1    Administer pentobarbital sodium solution (1 grain/mL ) to the jird by intraperitoneal (IP) injection at a rate of 0.5-0.7 mg/g b.wt. to produce general anesthesia.

2.2.2    Prepare the jird(s) by clipping the right ventral abdomen and swabbing the skin at that location with 70% ethanol.

2.2.3    Make a small laparotomy incision and insert the Brugia sp. IP using a sterile bulb pipette.

2.2.4    Close the laparotomy incision with an auto clip staple (or suitable alternative).

2.2.5    Monitor the jird(s) during recover from anesthesia.