Mass Spectrometry and Chromatography at Smith College
CONSULTATIONS
- Experimental design and planning
- Proper sample preparation for subsequent MS-based analysis
- Assistance with data interpretation
ASSISTANCE WITH SAMPLE PREPARATION OO (please note that the CFP does not provide reagents, kits or other materials)
- Sample clean-up and processing (extraction, desalting, precipitation, depletion, fractionation, proteolytic digest, etc.)
- Standard biochemical techniques (SDS-PAGE gel electrophoresis and staining, Western blotting, etc.)
- Affinity-based enrichment (antibody, immobilized metal affinity, etc.)
MASS SPECTROMETRY
Small Molecules (see Protocols for appropriate sample preparation):
- Electrospray ionization of acidic, basic, charged and polar molecules (direct infusion)
- Reverse phase liquid chromatography hyphenation (other stationary phases possible, must be supplied by researcher)
- MS and MS/MS
- Positive and negative ion detection
- Mass determination and quantitation (when standards are available)
Proteomics:
- Protein and peptide identification in purified samples or complex mixtures
- Protein Quantitation: Isobaric tags
- PTM identification
- Intact protein analysis
DATA ANALYSIS