Crayfish - Standard Saline (after van Harreveld, 1936)

1 Liter

2 Liters

3 Liters

NaCl

KCl

CaCl2 (anhydrous)

MgCl2• 6H2O

12.0 g/L

0.4 g/L

1.5 g/L

0.5 g/L

24.0 g

0.8 g

3.0 g

1.0 g

36.0 g

1.2 g

4.5 g

1.5 g

Mix these four salts with slightly less than the final amount of distilled water; then add the following salt and bring the solution to its final volume with additional distilled water:

NaHCO3

0.17 g/L

0.34 g

0.51 g

Aerate and mix the solution thoroughly. Check the pH; if necessary, add a few drops of dilute HCl or additional NaHCO3 to bring the solution to pH 7.4.

Crayfish - High K+ (60 mM)

Same as Crayfish - Standard Saline except:

NaCl

KCl

8.87 g/L

4.42 g/L

17.74 g

8.84 g

Crayfish - Low Ca++ (2.7 mM), High Mg++ (52 mM)

Same as Crayfish - Standard Saline except:

NaCl

CaCl2 (anhydrous)

MgCl2• 6H2O

6.8 g/L

0.3 g/L

1.0 g/L

Earthworm (Lockwood, 1961)

NaCl

KCl

CaCl2 (anhydrous)

NaHCO3

6.0 g/L

0.12 g/L

0.2 g/L

0.1 g/L

Cockroach (Prosser & Brown, 1961)

NaCl

KCl

CaCl2 (anhydrous)

MgCl2• 6H2O

10.93 g/L

1.57 g/L

0.85 g/L

0.34 g/L