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All equipment will be on the HHMI Scheduler at the link on the left side of this website or at https://www.science.smith.edu/resources/assess/login.php
Users will need to be granted access to use the equipment by Lou Ann Bierwert at email@example.com
Once access is granted, you will be able to sign in to the scheduler with your Novell username and password,
and schedule your use of the equipment, from any computer with internet access.
Scheduling is vital in order to avoid conflicts with other researchers and to document use of the equipment for
future funding and upgrades. Sincerest thanks in advance for your cooperation.
Image Instrument Location Application Qubit 2 Fluorimeter Ford 123
The Qubit Fluorimeter allows you to quantitate dsDNA, ssDNA, RNA, or protein specifically by measuring fluorescence bound to the specific components, giving more reliable quantitation and indication of contaminants than traditional spectrophotometry.
Agilent Bioanalyzer Ford 123 The Agilent Bioanalyzer allows for in depth detail of quantity and quality of RNA and DNA samples or libraries, with a breakdown of range and average fragment size. This type of information is critical in library prep for Next Generation Sequencing and other downstream applications. MiSeq Next Generation Sequencer by Illumina Ford 123
The MiSeq can sequence entire metagenomes, genomes or transcriptomes from multiple samples in a single run. With up to 25 million reads, 2 x 300bp paired end runs, and automated data storage and analysis in the BaseSpace cloud, sequencing doesn't get any easier than this:)
Full-spectrum UV-Vis absorbance analyses (220-750nm) for measuring absorbance of DNA, RNA, dyes, proteins and microbial cell culture OD; 1ul of sample is needed (we recommend using 2ul); Large dynamic range: 2-3700 ng/ul of dsDNA.
Molecular Devices Spectramax M5 Plate Reader
SpectraMax M5 / M5e is a dual-monochromator, multi-detection microplate reader with a triple-mode cuvette port and 6-384 microplate reading capability. Detection modalities include absorbance (UV-Vis), fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF) and luminescence (Lum).
Beckman DU 530 Spectrophotometer Ford 123
A totally affordable, completely modular UV/Vis Spectrophotometer which provides easy sample handling and operation, along with accurate measurement of all your samples
Applied Biosystems 3130xl
a multi-color fluorescence-based DNA analysis system with 16 capillaries operating in parallel for a wide range of sequencing and fragment analysis applications
Applied Biosystems 7300 Real Time PCR System Ford 123
This system performs a wide variety of applications including gene expression analysis, pathogen quantitation, SNP genotyping, and +/- assays that utilize internal positive controls
Real-Time PCR System
Ford 123 This system performs a wide variety of applications including gene expression analysis, pathogen quantitation, SNP genotyping, and +/- assays that utilize internal positive controls. Uses Fast-Plates and is is Fast-chemistry capable, with a few more features for master mix calculations etc. Herlmle centrifuge Refridgerated Ford 123
Bench top centrifuge, covers separation in 15ml / 50ml tubes, and 96-well plates.
Applied Biosystems Veritii Thermal Cycler
The Applied Biosystems Veritii Thermal Cycler can hold 0.2ml tubes or 96 well plates. It has a Veri-Flex block allowing one to use up to 6 different annealing temperatures for reaction optimization. MJ Research PTC-200
Peltier Thermal Cycler
Thermal cycler can hold 0.2ml and 0.65ml PCR tubes, can operate two PCR programs simultaneously;
Interchangeable Alpha unit sample blocks are designed for frequent change
Spot Bot Microarray Printer Ford 123 Spots down oligos or cDNA in determined grid from 96 or 284 well plates. Capable of printing up to 50,000 spots on a single microarray slide. GenePix Personal
Microarray scanner for microarrays, tissue and cell arrays, and fluorescence studies
Typhoon 9200 Variable Mode Imager
Versatile imaging system handles chemiluminescence and flourescence in gel sandwiches, agarose and polyacrylamide gels, membranes,
microplates, and even microarrays
EpiChem Transilluminator Ford 123 Versatile imaging system handles chemiluminescence and flourescence in gel sandwiches, agarose and polyacrylamide gels, membranes,
microplates, and even microarrays. Unlike the Typhoon, gel prep(cutting out bands, etc) can be done with this instrument.
BioRad crosslinker used for linking nucleic acids to membranes and microarray slides
Rotary incubator can be used for still or rotary incubation of tubes and bottles.
BioRad Biolistic PDS 1000 He System
The biolistic PDS-1000 He™ system uses a burst of high-pressure helium gas to accelerate micron-size nucleic acid-coated gold or tungsten microparticles to velocities necessary to transfect cells, tissues, or organelles without dependence or interaction with the cell surface.