Mass spectrometry technologies have a broad application in projects in the biochemistry, biology, chemistry and proteomics research communities. As a general overview, the mass spectrometry resources available in the Center for Proteomics are outlined below. We will work with the users of the center on an individual basis to provide a customized mass spectrometry approach for their particular field of interest.
Types of Mass Spectrometry Experiments we can perform:
- Full- Scan Mass Spectra (Molecular Weight Information)
- Selected Ion Monitoring (SIM) for monitoring selected ions
- Full Scan MS/MS and MSn experiments to probe the structure of ions
- ZoomScan, a high resolution, narrow range scan that can be used to determine charge state of peptides and ionization state of organometallics
- Selected Reaction Monitoring (SRM) for LC/MS quantitative experiments and positive compound identification
Types of Ionization probes available:
Electrospray ionization (ESI).
Used to analyze more polar compunds such as amines, peptides, oligonucleotides and proteins. Range of molecular weights that can be analyzed is 50u to greater than 100, 000u due to multiple charging.
Atmospheric pressure chemical ionization (APCI).
Used to analyze compounds of medium polarity that have some volatility. Range of molecular weights is 50u to 2000u. Very robust ionization technique that is less dependant on changes in buffer or buffer strength.
Nanospray ionization (NSI).
Essentially the same as ESI except it uses lower flow rates than ESI (10 to 1000 nL/min). Three types of NSI probes are available, static NSI, dynamic NSI and Packed-Tip NSI.
Information for new MS users:
Please contact the center to determine what kind of analysis you need, for consultation and further information.