Overview
2-D gel electrophoresis is a separation technique that allows you to resolve complex mixtures of proteins. In the first dimension, the proteins are separated by their isoelectric point (pI) and in the second dimension, the proteins are separated by their molecular weight. The 2-D workflow using Bio-Rad apparatus is outlined below and the center has the equipment to run mini-gels, midi-gels and large gels.
Tutorials
Tutorial: 2-D Gel electrophoresis for Proteomics, University of Wales. An excellent overview of the biochemistry behind 2-D gel electrophoresis.
Tutorial: How to run 2-D gels, Bio-Rad. How-to-videos from Bio-Rad with tips for 2-D gel electrophoresis techniques, like sample preparation and how to cup load a 2-D gel.
Protocols for 2-D gel electrophoresis
Protocols for protein extraction and sample preparation
Tips to prevent streaking on 2-D gels
2-D databases
2-D hunt , searchable database for virus and bacterial proteomes, University of Aberdeen
Human 2-D PAGE database, Danish Center for Human Genome Research