Immunoscreening with human sera
from infected patients.
| CLONE NAME and OTHER NAMES | LIBR-ARY | SCREENING TOOL | HOMOLOG/IDENTITY | RECOMB AG SIZE / LOCALIZATION / STAGE | SPECIES | COMMENTS | INVESTIGATOR | REF |
| Ovcol-1
OvMBP/29 |
Ad cDNA | PCR
Hu Ov sera & not Wb sera |
small nematode collagen | 9.7 kDa predicted, 14.2 kDa under reduced SDS-PAGE, immunolocalized to the cuticles of adults and uterine microfilariae | Ov | Perferentially recognized by IgG3 from PI individuals from Ecuador | Stewart/Bradley | 23 |
| pOG11 | Mf cDNA
of Og |
Pooled INF, hybridization with Ov Ad cDNA | repetitive antigen | Found in high MW fraction of Ag, immunolocalized to muscle and specific membrane layers | Ov, Og | Recognized more strongly by patients with sowda | Miller/
Catmull |
30 |
| OvMBP20/11 | Ad cDNA | Hu Ov sera & not Wb sera | homologous to BM20 SEC-1 from Globodera pallida and several C.e. ORFs F02A9.2 retinol binding protein | Body wall of adult, mf, L3 and L4. Detected also in ES | Ov
Av Bm |
diagnostic Ag,
Ab to OvMBP20/11 in INFPI, proliferation to OvMBP20/11 in PI+INF |
Bradley/Maizels
Bradley/Nutman |
37
38 |
| Ov39 | Ad cDNA | human serum from infected patients | F40E10.5 of C.e. is a possible homolog | 22 kDa protein in adult | Ov | is immuno-logically cross-reactive with 44 kDa component of the retinal pigment epithelium cells | Taylor/Braun | 35, 12 |
| PG3 | ? | Pooled sera from patients with sowda | collagen | Ov | Garate/Parkhouse | 24 | ||
| Ov16
? |
Ad cDNA | Affinity purified human antibodies from Ov sera |
similar to phosphatidylethanol-amine binding protein of vertebrates |
16 kDa, hypodermis cuticle uterus in adult worms | Ov | diagnostic Ag | Lobos/Weiss
Erttmann/Gallin |
2
39 |
| OvG15 | Ad cDNA | Hu Wb SERA | HSP 70 homologous to BmHSa and F26D10.3 from C.e. | 70 kDa | Ov | preferentially recognized by mf- | Rothstein / Rajan | 4 |
| Og 11 | Mf (Og) | Hu Ov sera | 87 bp repeat | Og | Catmull/Miller | 7 | ||
| OV33
OC 3.6
OV5B/3B |
Ad cDNA
Ad cDNA
Ad cDNA |
Hu Ov sera
Hu Ov sera vs. other filarial sera R anti-Ov adult |
Aspartyl protease inhibitor homologous to Ce ORF celf32a5 |
130 kDa b-gal fpp 70/85 kDa MBP fpp |
Ov Di Bm
Ov Ov Di |
diagnostic Ag | Lucius/Donelson
Chandrashekar/ Weil Nkenfou/Perler |
9
6 * |
| Onchoag-1 | Ad cDNA | Hu Ov sera | myosin heavy chain | 200 kDa, muscle in adult | Ov | Erondu/Donelson | 10 | |
| OV 22/31M | Ad cDNA | Hu Ov sera & not Wb sera | 20 kDa protein, adult | Ov | Bradley/Maizels | 14 | ||
| OW10 | Ad cDNA | Hu Ov sera | (?) 3 kDa (90 bp ORF) | Ov | T-cell epitope identified | Colina/Perler/
Nutman |
19 | |
| aS1 | Ad cDNA | Hu Sow and not GEN or END | related to human chemokine | ov | IgG3 reactivity was restricted to sowda | Erttmann/Gallin | 40 | |
| OvGalBP | Ad CDNA | Hu anti-Loa loa | vertebrate galactoside-binding protein | 32 kDa | recognized by INF and not by PI | Klion/Donelson | 41 | |
| OV 103 | Ad cDNA | infected Chimpanzee sera | some homology to C.e. ORF ZC410.5 | 15 kDa, in hypodermis & basal cuticle (ad) & surface (mf) | Ov | Ab anti-OV 103 kill mf in vitro (ADCC) | Lustigman/Prince | 11 |
| CLONE NAME and OTHER NAMES | LIBR-ARY | SCREENING TOOL | HOMOLOGY/IDENTIT | RECOMB AG SIZE / LOCALIZATION / STAGE | SPECIES | COMMENTS | INVESTIGATOR | REF |
| Ov64
Ov18 GAAP |
L3 cDNA
? L3 cDNA |
PI sera from Liberia/Ecuador
? PCR based on protein sequence |
homologous to Av18 and Di 20/22
as above |
highly expressed SL1 trascript
in Bm and Ov, temporal expression during molting, ES
as above |
Ov, Av, Di | Recognized by 85-95% PI and INF from Cameroon. 40% protection in mice | Joseph/Lustigman
Wisnewski/Grieve Wu/Bianco |
*
* * |
| OV73K
Ov4.21 Oveg1 |
L4 cDNA
L3 cDNA Ad cDNA |
PI sera from Liberia/Ecuador
PI vs. INF Ecuador Serum from MF +++ patients |
putative substrate for TGase during
molting
|
In secretory vesicles and glandular esophagus of molting L3 and L4, in cuticles of L4 and Ad. In eggshell of developing mf in the uterus. | Ov | Recognized by 90-100% of INF and
PI from Liberia, 67% of INF and PI from Ecuador.
13% reduction of Ov larvae recovery in mice |
Joseph/Lustigman
McCarthy/Nutman Tume/McKerrow
|
*
* 27 |
| OI5/OI3 | Ad cDNA | PI vs. INF | 37 and 45 kDa proteins Adutl, mf and L3 | Ov | 200 kDa size of the native protein | Perler/Nutman/
Tuan |
8 | |
| E1 | Ad cDNA | PI serum | brain ankyrin | 90 kDa on SDS-PAGE,
localized to the neuronal bodies nerve ring and extra cellular clefts of the basal labyrinth |
Ov | preferentially recognized by PI sera | Erttmann/Gallin | 25 |
| OvB8
Ov87 Aldolase Actin |
L3 cDNA | PI sera from Liberia/Ecuador | Ov | recognized by PI and INF | Joseph/Lustigman | * |
Immunoscreening with anti-X-irradiated L3 sera
| CLONE NAME and OTHER NAMES | LIBR-ARY | SCREENING TOOL | HOMOLOG/IDENTITY | RECOMB AG SIZE / LOCALIZATION / STAGE | SPECIES | COMMENTS | INVESTIGATORS | REF |
| OvB20 | Ad cDNA | differential immunoscreening using cattle sera against X-L3 of Ol vs. infected cows | Has possible C.e. homolog ORF T17H7.4 | 52 and 65 kDa expressed in embryo to L4 but not in adults | Ov | recombinant protein confers 49-60% protection in jirds against Av | Tuan/Bianco
Abdel-Wahab/Bianco |
26
42 |
| OvL3-1 | Ad cDNA | human serum raised against radiation attenuated Ov L3 | LIM domain protein homologous to AvL3-1 and F42G4.e from C.e. | 50 kDa on immunoblot | Ov, Av | recombinant protein offers no protection in the Av jird model | Oberlander/ Lusius | 33
34 |
| OV 7
OC9.3 OvMBP/10 |
Ad cDNA | Chimp anti-X-L3
Hu Ov sera vs. other filariae sera Hu Ov sera & not Wb sera |
Cystatin homologous to B.m. and C.e.ESTs | 17 kDa, in hypodermis basal cuticle (L3, Ad) & eggshell (uterine mf) | Ov, Bm
Av |
recombinant protein confers 36-49% protection in mice aginst Ov | Lustigman/Prince
Chandrashekar/ Weil Bradley/Maizels |
13
6 14 |
| Chitinase
|
L3 cDNA of Av | sera of
vaccinated jirds |
homologous to Ov and Bm chitinase | 52/75 kDa
glandular esophagus in L3 and uterine mf in female worms |
Av,Ov, Bm, Bp, Di | Adam/Lucius | 45 | |
| Tropomyosin 1, LsTM1
Tropomyosin 2, LsTM2 |
L3 cDNA of Ls | serum from vaccinated rabbit anti Ov | Ov Tropomyosin | Ls, Ov | MacLennan/Taylor | 47 |
Immunoscreening with Mab's or other specific antibodies
| CLONE NAME and OTHER NAMES | LIBRARY | SCREENING TOOL | HOMOLOGY/IDENTITY | RECOMB AG SIZE / LOCALIZATION / STAGE | SPECIES | COMMENTS | INVESTIGATORS | REF |
| M2f.e (OVMS18) | Ad cDNA | Anti-Di mf 18 kDa surface protein | 18 kDa mf surface | Ov Di other filariae | Dinman/Scott | 5 | ||
| OVIF
OV1CF |
Ad cDNA |
?
Mab OV-1 |
intermediate filament protein (IF) has several nematode homologs | 70 kDa
immunolocalized to muscle and specific membrane layers 65-70 kDa, circulating Ag, crossreactive with keratin |
Ov
Ov |
Zang/Miller
Chandrashekar/ Weil |
28
43 |
|
| 2A4.1 | Mab 2A4E9 | 14 kDa, surface of mf | Og, Ov | phenylalanine-rich protein | Conraths/
Parkhouse |
1 | ||
| 2A5.1 | Mab 2A5B9 | myosin/paramyosin | 92 kDA | Og,Ol | Conraths/
Parkhouse |
1 |
Immunoscreening with rabbit sera raised against L3
and/or L4
| CLONE NAME and OTHER NAMES | LIBR-ARY | SCREENING TOOL | HOMOLOG/IDENTITY | RECOMB AG SIZE / LOCALIZATION / STAGE | SPECIES | COMMENTS | INVESTIGATORS | REF |
| RAL-1 | Ad cDNA | Rabbit anti-Ov L3 | calreticulin
homologous to S25851 from C.e. |
42 kDa, adult and L3 | Ov | stimulates T-cell prolifaration,
Ab in 86% INF |
Unnasch / Greene | 20 |
| RAL-2
Ov17 |
Ad cDNA
Ad cDNA |
Rabbit anti-Ov L3
Hu Ov sera & not Wb sera |
homologous to Brugia SXP-1 and C. e. EST CEESG72R | 17 kDa, hypodermis of adult worms and L3 | Ov | Ab response in INF is irreversibly correlated with ocular pathology | Gallin / Unnasch
Bradley/Unnasch |
21
44 |
| EF-1a | Ad cDNA | Rabbit anti-L3 | translation elong factor 1-A, homologous to C.e. EST CEESE09R | Ov | message 70% abundant as actin | Alarcon / Donelson | 3 | |
| Mov2
Ov-20, Ov-9 |
Ad cDNA | Rabbit anti-L3 | 18/22 KDa protein, in annulae of L3 and uterine wall of female worms. All stages. Secreted by L3 and adult worms of A.v. | Ov, Av | 55% reduction in adult worm recovery in the Av model | Jerkins/Bianco | 46 | |
| Mov14
OvTROP |
Ad cDNA | Rabbit anti-L3 | tropomyosin | 40 kDa in ES of larvae
42 kDa in L3 extracts |
Ov, Tc | Important antigen in immunity against Trichostrongylus in sheep. 48-62% reduction of Ov mf survival in mice | Jenkins/Bianco
Taylor/Bianco |
47 |
| Ov9M | Ad cDNA | Rabbit anti-L3 & L4
|
calponin | 45 kDa, in the longitudinal muscles of larvae and adult worms | recognized by 40-60% of INF and PI | Irvine/Lustigman | 28 |
Rational screening for molecules important to parasite
| CLONE NAME and OTHER NAMES |
LIBRARY |
SPECIES |
COMMENTS | HOMOLGY/IDENTITY | INVESTIGATOR | REF | |||||
| OvGlt | Ad cDNA | PCR | glutamate transporter homologous to D86740 from C.e. | Ov Ce | Radice/
Lustigman |
29 | |||||
| OVB2 | Ad gemonic | HYBRID TO Bm cDNA FOR 65KD AG | ASPARTYL t-RNA SYNTHETASE | Ov | NO RNA IN AD MF | Kron/
Unnasch |
15 | ||||
| OVGS1/OVGS2 | Ad gemonic | ASCARIS MSP cDNA | MAJOR SPERM PROTEIN | Ov | MSP IS 5% SPERM PROT; CYTOSKEL | Scott/
Ward |
16 | ||||
| ov para | Ad cDNA | Rab a-Di paramyosin | PARAMYOSIN | 98 kDa; 848 Aa | Ov Di Ce | 99% ID to Di para-myosi | Limberger/
McReynolds |
17 | |||
| OVT1 | Ad cDNA | Ag to embryo associated proteins | paramyosin like protein | 230 kDa
expressed in late morula/early blastocyst stage and hypodermal cells of larvae and adults |
Ov | Triteeraprapab/
Scott |
36 | ||||
| D 1-3 | Erttmann / Gallin | * | |||||||||
| ACTIN 1A / 2B | Ad | hu B-ACTIN cDNA | ACTIN | 41 kDa (CALCULATED) | Ov | Zeng/Donelson | 22 | ||||
| OVCP | Ad, L3 cDNA | PCR | cysteine protease | Ov, Oo | Lustigman/
Brotman |
48 | |||||
| chitinase | cDNA | PCR from protein sequence | homolous to chitinase of Bm mf | 53 kDa | Ov | L3 specific | Wu/Bianco | 42 | |||
| nicotinic acethylcholine receptor | Ajuh/Edwang | ||||||||||
| CyP1
CyP2 |
Ad and L3 cDNA | Bm CyP probe and antibodies | cyclophilin | CyP1-150 kDa
CyP2-18 kDa |
Ov, Bm, Di | Carlow et al. | * | ||||
| PTG08 | Ad cDNA | Ab to trans-glutaminase | TSA | hypodermis in adult worms | Ov | Chandrashekar/
Weil |
* | ||||
| GST-2
P24 |
Ad cDNA | polyclonal Ab anti-GST | Ov | Salinas/Taylor | * | ||||||
| OvA3 | Ad cDNA | PCR | lin 12 related | Ov | Bialonslu/Zipfel | * | |||||
| OVSOD1
OVSOD2 OVSOD3 |
Ad cDNA | PCR | superoxide dismutase | Ov | A Cu/Zn SOD | Henkle/
Walter |
18 | ||||
| OVGST1
OVGST2 OVGST3 |
Ad cDNA | Ab | glutathione S-transf-erase | Ov | Henkle | * | |||||
| OVGR1 | Ad cDNA | PCR | glutathione reductase | Ov | Muller | * | |||||
| OVPTK3
OVPTK2 |
Ad cDNA | PCR | protein tyrosine kinase | Ov | Henkle | * | |||||
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* not published