Available Onchocerca volvulus cDNA and Genomic Libraries

 

This list is not complete because new libraries are constantly being made. If you would like to have your library(ies) listed on this page please e-mail Véronique Vouille at vvouille@math.smith.edu

For information about other filarial parasite DNA libraries, check out the List of Filarial DNA Libraries provided by the Filarial Genome Network.

Go back home

Onchocerca volvulus microfilaria cDNA Library

Contact/Investigators

Steven A. Williams & Michelle Lizotte-Waniewski
Clark Science Center
Smith College
Northampton, MA 01063, USA
FAX: 413 585 3786
TEL: 413 585 3826
EMAIL: swilliam@sophia.smith.edu

Library Information

Name: Onchocerca volvulus microfilaria cDNA library (reference number SAW98MLW-OvMF)
Organism: Onchocerca volvulus
Disease: River Blindness
Vector: Lambda Uni-ZAP XR (Lambda ZAP cut with EcoRI and XhoI - Stratagene)
Cloning Sites: EcoRI (5' end) and XhoI (3' end)
Host Strain: XL-1 Blue MRF' strain
Unamplified Titer: 7.8 X 104 pfu/ml
Amplified Undiluted Titer: ~ 1010 pfu/ml
Average Insert Size: ~ 930 bp
Background: 28% non-recombinants
Availability: The library is available from Dr. S.A. Williams, email genome@smith.edu

Onchocerca volvulus L2 cDNA library

Contact/Investigators

Steven A. Williams & Michelle Lizotte-Waniewski
Clark Science Center
Smith College
Northampton, MA 01063, USA
FAX: 413 585 3786
TEL: 413 585 3826
EMAIL: swilliam@sophia.smith.edu

Library Information

Name: Onchocerca volvulus L2 cDNA library (reference number SAW98MLW-OvL2)
Organism: Onchocerca volvulus
Disease: River Blindness
Vector: lambda UniZap XR (Lambda ZAP cut with Eco RI and Xho I - Stratagene)
Cloning Sites: EcoRI (5' end) and XhoI (3' end)
Host strain: XL1-Blue MRF'
Unamplified Titer: 7.3 X 104 pfu/ml
Amplified Undiluted Titer: ~ 1010 pfu/ml
Average Insert Size: ~ 1030 bp
Background: 30% non-recombinant
Availability: The library is available from Dr. S.A. Williams, email genome@smith.edu.

Onchocerca volvulus L3 infective larva cDNA

Contact/Investigator

Steven Williams
Clark Science Center
Smith College
Northampton, MA 01063, USA
FAX: 413 585 3786
TEL: 413 585 3826
EMAIL: swilliam@sophia.smith.edu

Library Information

Name: Onchocerca volvulus infective larva (L3) cDNA library (reference number SAW94WL-OvL3)
Organism: Onchocerca volvulus
Disease: River Blindness
Vector: lambda UniZap XR (Lambda ZAP cut with EcoRI and XhoI - Stratagene)
Cloning Sites: EcoRI (5' end) and XhoI (3' end)
Host strain: XL1-Blue MRF'
Unamplified Titer: 1.8 X 105 pfu/ml
Amplified Undiluted Titer: ~ 1010 pfu/ml
Average Insert Size: ~ 900 bp
Background: ?% non-recombinant
Detailed description: mRNA was prepared from third stage infective larvae of Onchocerca volvulus isolated from mosquitoes 10 days after infection and converted to double stranded cDNA using reverse transcriptase and oligo(dT) followed by RNase H and DNApol I. The library had 1.8 x 10E5 independent recombinants and average insert size was 900 base pairs. The library was constructed by Wenhong Lu.
Availability: The library is available from Dr. S.A. Williams, email genome@smith.edu.

Onchocerca volvulus molting L3 cDNA Library

Contact/Investigators

Steven A. Williams & Michelle Lizotte-Waniewski
Clark Science Center
Smith College
Northampton, MA 01063, USA
FAX: 413 585 3786
TEL: 413 585 3826
EMAIL: swilliam@sophia.smith.edu

Dr. Sara Lustigman
New York Blood Center
310 East 67th Street
New York, NY 10021
TEL: (212) 570-3119
FAX: (212) 570-3180

Library Information

Name: Onchocerca volvulus L3/L4 transition cDNA Library.
Organism: Onchocerca volvulus
Disease: River Blindness
Vector: Lambda Uni-ZAP XR (Lambda ZAP cut with EcoRI and XhoI - Stratagene)
Cloning Sites: EcoRI (5' end) and XhoI (3' end)
Host Strain: XL-1 Blue MRF' strain
Unamplified Titer: 2.2 X 106 pfu/ml
Amplified Undiluted Titer: ~ 1010 pfu/ml
Average Insert Size: ~ 1100 bp
Background: 7% non-recombinants
Availability: The library is available from Dr. S.A. Williams, email genome@smith.edu.

Onchocerca volvulus adult female cDNA Library

Contact/Investigators

Steven A. Williams & Michelle Lizotte-Waniewski
Clark Science Center
Smith College
Northampton, MA 01063, USA
FAX: 413 585 3786
TEL: 413 585 3826
EMAIL: swilliam@sophia.smith.edu

Library Information

Name: Onchocerca volvulus adult female cDNA library
Organism: Onchocerca volvulus
Disease: River Blindness
Vector: Lambda Uni-ZAP XR (Lambda ZAP cut with EcoRI and XhoI - Stratagene)
Cloning Sites: EcoRI (5' end) and XhoI (3' end)
Host Strain: XL-1 Blue MRF' strain
Unamplified Titer: 7 X 105 pfu/ml
Amplified Undiluted Titer: ~ 1010 pfu/ml
Average Insert Size: ~ 1150 bp
Background: 5% non-recombinants
Availability: The library is available from Dr. S.A. Williams, email genome@smith.edu.

Onchocerca ochengi L3 cDNA library

Contact/Investigator

Steven A. Williams & Michelle Lizotte-Waniewski
Clark Science Center
Smith College
Northampton, MA 01063, USA
FAX: 413 585 3786
TEL: 413 585 3826
EMAIL: swilliam@sophia.smith.edu

Library Information

Name: Onchocerca ochengi L3 cDNA library
Organism: Onchocerca ochengi
Disease: Filarial skin disease of cattle in Africa
Vector: lambda UniZap XR (Lambda ZAP cut with EcoRI and XhoI - Stratagene)
Cloning Sites: EcoRI (5' end) and XhoI (3' end)
Host strain: XL1-Blue MRF'
Unamplified Titer: 4.0 X 105 pfu/ml
Amplified Undiluted Titer: ~ 1010 pfu/ml
Average Insert Size: ~ 1000 bp
Background: 8% non-recombinants
Availability: The library is available from Dr. S.A. Williams, email genome@smith.edu.

back to top
This page was last modified on January 26th 2000