Labs are in Sabin Reed 314, on Tuesdays from 1:10 - 4:50 and other times as required.
9/7/2004 Orientation, Grouping, Check-in, Calibrate micropipetters and Prepare buffers and for the next labs
Salivary a-amylase purification and characterization
9/14/2004 a-Amylase Purification + Total protein determination
9/21/2004 Activity and Protein Determinations
Report 1: results + discussion on the two first labs, due on 9/28/2004
9/28/2004
SDS-Polyacrylamide Gel Electrophoresis and Western Blot
10/5/2004
Immunostaining
Report 2: Full lab report (Abstract + introduction + results + discussion) on a-Amylase purification due on 10/19/2004
Colorimetric Identification of Unknown Sugars
10/19/2004 Colorimetric
Identification of Unknown Sugars
No Report (Results reported in lab)
Red muscle mitochondria cytochrome c purification and characterization
10/26/2004
Isolation of Cytochrome c from Red Muscle Mitochondria
11/2/2004 NoLab
11/9/ 2004 Chromatography I: Ion exchange
11/16/2004 Chromatography II: Gel filtration
11/23/2004 Determining
Purity, Yield and "Specific Activity"
Report 3: Full lab report (Abstract + introduction + results + discussion)
on the four labs on cytochrome c, due on 11/30/2004
Independent Projects: Determination of Glycemic Index
Values
11/30/2004 Independent
Projects: Determination of Glycemic Index Values
12/7/2004 " " "
" " "
Report 4: Full lab report on Independent Project due on 12/14/2004
12/14/2004 Oral presentations
Note: You are expected to keep a complete record of every experiment in
your notebook. Notebooks will be checked after each of the three first labs
and graded at the end of the semester.
All work will be graded on a total of 100 points, with the following weights for each assignment:
Report 1: 10%
Report 2: 10%
Report 3: 20%
Report 4: 20%
Oral presentations: 20%
Lab notebook: 20%
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