Labs are in Sabin Reed 314, on Tuesdays from 1:10 - 4:50 and other times as required, except for the bioinformatics labs that will take place in Burton 302.
9/10/2002 Orientation, Grouping, Check-in & Prepare buffers and Calibrate micropipetters for the next labs
Part I: Introduction to Protein Structure Prediction using web based tools
9/17/2002
Session 1
Handling databases, sequence alignment and Blast searches
9/24/2002
Session 2
Exhaustive evaluation of secondary structure prediction tools
Report 1: Web page due on 10/01/2002
Part II: Salivary a-amylase purification and characterization
10/01/2002
a-Amylase Purification + Total protein determination
10/08/2002 FALL BREAK
10/15/2002 Activity Determinations
Report 2: results + discussion on the two first labs, due on 10/22/2002
10/22/2002
SDS-Polyacrylamide Gel Electrophoresis and Western Blot
10/29/2002
Immunostaining
Report 3: Full lab report (Abstract + introduction + results + discussion) on a-Amylase purification due on 11/05/2002
Part III: Red muscle mitochondria cytochrome c purification and characterization
11/05/2002
Isolation of Cytochrome c
from Red Muscle Mitochondria
11/12/2002 Chromatography I: Ion exchange
11/19/2002 Chromatography II: Gel filtration
11/26/2002 No Lab
12/03/2002
Determining Purity, Yield and "Specific Activity"
12/10/2002 Oral presentations
Report 4: full lab report (Abstract + introduction + results + discussion) on the four labs about Cytochrome c, due on 12/13/2002
Note: Apart from the lab reports, you are expected to keep a complete record
of every experiment in your notebook that will be checked after the three
first labs and graded at the end of the semester.
All works will be graded on a total of 100 points, with the following weights for each assignment:
Report 1: 10%
Report 2: 10%
Report 3: 20%
Report 4: 20%
Oral presentations: 20%
Lab notebook: 20%
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Last modified on August 8, 2002 
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