Biochemistry Laboratory

353 Fall, 2004

Biochemistry Laboratory 353 materials available:

• Lab reports
• Notebooks & Data Recording
• Laboratory Syllabus
• Calibrate micropipetters
• Preparing a buffer
• Lab 1: Purification and Characterization of Human Salivary a-Amylase
• SDS gel Electrophoresis
• Western Blotting
• Immunostaining
• Lab 2: Identification of an Unknown Sugar
  
• Lab 3: Purification of Cytochrome c
• Purification Protocol
• Protein and Cytochrome c quantification
• Lab 4: Independent Projects: Determination of Glycolytic Index Values
  
• Lab History
• Bradford Protein Assay
• Buffers and Buffering
• Buffer Theory
• How to prepare a buffer
• Common buffers
• Link to Biochemistry 352

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