Molecular weight standards used for SDS-PAGE:
The molecular weight standards used in the SDS-polyacrylamide gel electrophoresis and Western blotting were, "Color Markers, Wide Range" (Sigma).
The dye attachment increases the apparent molecular weights of the modified proteins. The column headed "Apparent Molecular Weight (in Daltons)" is the estimate provided by Sigma for their apparent molecular weights when used as standards in SDS-PAGE. My personal experience is that the apparent molecular weights actually determined by SDS-PAGE are usually somewhere between the actual protein molecular weights and the estimates from Sigma. Life is never simple, is it?
|Color||Apparent Molecular Weight
|Myosin, Rabbit Muscle||205,000||Blue||225,000|
|Albumin, Bovine Serum||66,000||Pink||73,000|
|Ovalbumin, chicken egg||45,000||Yellow||50,000|
|Carbonic Anhydrase, bovine||29,000||Orange||31,000|
|Trypsin Inhibitor, soybean||20,000||Green||26,000|
|Lactalbumin, bovine milk||14,200||Purple||20,000|
|Aprotinin, bovine milk||6,500||Blue||10,000|