CMBS services

We will run your purified sequencing samples using the Applied Biosystems 3100 Genetic Analyzer. You need to follow the rules listed below to submit your samples and retrieve your data.

The current cost for each ready-to-sequence sample is $1.50. It is relatively low because the reagents are kindly supplied by Prof. Steven Williams’ lab for now. It may change in the future.

Each lab is responsible for purification of your DNA sequencing samples before submitting to the CMBS.
Put PI initials plus sample’s serial number (must be 4 digits unless you’ve passed 9999, then use 5 digits) on the top of each tube containing to-be-sequenced sample, such as “LAK0123”.
Every time you submit new batch of samples, the serial number of the sample must be sequential from the last batch.
There are two boxes in the freezer (the higher one) in SR406. They are labeled as “Sequences To Be Run on 3100 Box 1” and “Box 2”. Put 8 samples in a row only. Put samples in numerical order. Please fill out the Box 1 before put into Box 2.
Fill out the order form which is in the binder on the top of the freezer (the lower one, next to the higher one).
The CMBS will run sequencing samples on the “first come, first served” basis.
We are using the ABI3100 DNA Genetic Analyzer to run our sequencing samples. Because 16 samples are injected to the capillaries simultaneously, if only a few samples are ready to be sequenced, the CMBS will wait for more samples coming and then start the run.
After samples have been run and sequence data have been extracted, the CMBS will transfer the data to your DROPBOX in the SCIENCE.DATAVOL server. An email from cmb@email.smith.edu will be sent to the PI or whoever is in charge, to notify the sequence drop-off.
Each lab should be responsible for having enough room for sequencing drop-off in your DROPBOX.
Currently the CMBS doesn’t have a second place to store your sequence data. The sequence data will be trashed after certain time (for now, it’s 3 months) in order to keep the sequencer function. You should be responsible for your data storage.

We will run your post-PCR samples using the Applied Biosystems 310 Genetic Analyzer. The current cost for each sample is $2.00. It may change in the future.

We also provide the instruments for the following experiments. You need to contact cmb@smith.edu to get training and make reservation before usage. Click here for more information on these instruments.